The biggest threat - the Hollywood Casino in Columbus - opened Oct. 8. Another horse track in Columbus also recently installed slot machines.
Now, officials from Mountaineer and Wheeling Island are telling lottery officials they are starting to see signs of impact.
"Columbus was their big draw," lottery director John Musgrave said. "They're very concerned, and of course we're concerned and we're trying to work with them to help any way we can."
The competition from Ohio isn't the only cloud looming over the Lottery Commission.
Pennsylvania recently cleared the way to open a new casino at the Nemacolon resort, about a 30-minute drive north of Morgantown.
Maryland voters are being asked to authorize table games in the November election. That could threaten revenues at the Hollywood Casino in Charles Town. It is West Virginia's biggest gambling operation by far.
A recent report financed by Maryland table games supporters found that legalizing table games there could divert $1.2 to $1.5 billion from Charles Town to Maryland.
Penn National Gaming, which owns the Charles Town casino, has spent close to $20 million on advertising to persuade voters to reject the Maryland table games measure.
West Virginia lottery officials also are hoping for a "no" vote.
"We're hoping that the folks over there will vote down that issue because it will have an impact on our facility in Charles Town," Musgrave said. "We do have concern."
Contact writer Jared Hunt at jared.h...@dailymail.com or 304-348-5148.